Show simple item record

dc.contributor.authorMorales-Amador, Adrián
dc.contributor.authorMolina-Miras, Alejandro
dc.contributor.authorLópez-Rosales, Lorenzo
dc.contributor.authorSánchez-Mirón, Asterio
dc.contributor.authorGarcía-Camacho, Francisco
dc.contributor.authorSouto, María L.
dc.contributor.authorFernández, José J.
dc.date.accessioned2021-07-30T07:01:53Z
dc.date.available2021-07-30T07:01:53Z
dc.date.issued2021-07-29
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10835/12082
dc.description.abstractThe demand for valuable products from dinoflagellate biotechnology has increased remarkably in recent years due to their many prospective applications. However, there remain many challenges that need to be addressed in order to make dinoflagellate bioactives a commercial reality. In this article, we describe the technical feasibility of producing and recovering amphidinol analogues (AMs) excreted into a culture broth of Amphidinium carterae ACRN03, successfully cultured in an LED-illuminated pilot-scale (80 L) bubble column photobioreactor operated in fed-batch mode with a pulse feeding strategy. We report on the isolation of new structurally related AMs, amphidinol 24 (1, AM24), amphidinol 25 (2, AM25) and amphidinol 26 (3, AM26), from a singular fraction resulting from the downstream processing. Their planar structures were elucidated by extensive NMR and HRMS analysis, whereas the relative configuration of the C-32®C-47 bis-tetrahydropyran core was confirmed to be antipodal in accord with the recently revised configuration of AM3. The hemolytic activities of the new metabolites and other related derivatives were evaluated, and structure–activity conclusions were established. Their isolation was based on a straightforward and high-performance bioprocess that could be suitable for the commercial development of AMs or other high-value compounds from shear sensitive dinoflagellates.es_ES
dc.language.isoenes_ES
dc.publisherMDPIes_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectamphidinoles_ES
dc.subjectAmphidinium carteraees_ES
dc.subjectdinoflagellate microalgaees_ES
dc.subjectphotobioreactores_ES
dc.subjecthemolysises_ES
dc.titleIsolation and Structural Elucidation of New Amphidinol Analogues from Amphidinium carterae Cultivated in a Pilot-Scale Photobioreactores_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://www.mdpi.com/1660-3397/19/8/432es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


Files in this item

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional