Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds
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Sales, Rafael; Cerón-García, María del Carmen; Navaro-López, Elvira; González-López, Cynthia Victoria; Yumi Tsuzuki, Mônica; [et al.]Fecha
2020-03-06Resumen
Extraction of carotenoids and fatty acids from microalgae is a technological bottleneck in processing. An
improved extraction process was developed to scale the production of these bioproducts from
Nannochloropsis gaditana. Different cell disruption methods were evaluated in terms of carotenoids
release. Ethanol was substituted by isopropyl alcohol in a three-component solution of water:isopropyl
alcohol:hexane (WIH), in which the extracts were separated by solution partitioning. This resulted in
higher carotenoid and fatty acid recovery yields if compared to the standard method. The extraction
method was replicated on a pilot scale obtaining similar carotenoid recovery yields, higher than those of
the standard method. Although fatty acid recovery was lower than that of the small-scale tests, yields
above 85% were obtained. This demonstrated that the method was scalable for the extraction of high-
value products from microalgae up to 10 L reactor volume. The use of isopropyl...