Effect of the Application of Hydrolysate of Chlorella vulgaris Extracted by Different Techniques on the Growth of Pelargonium × hortorum
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Author/sBayona-Morcillo, Pedro José; Gómez Serrano, Cintia; González López, Cynthia Victoria; Massa, Daniele; Jiménez Becker, Silvia
The extraction method used to obtain biologically active compounds from microalgal biomass may affect the biostimulant capacity of the microalgae. The objective of this assay was to determine the most efficient extraction method to release the active components of the biomass of Chlorella vulgaris (C. vulgaris). Plantlets of Pelargonium × hortorum were grown in a greenhouse and five treatments were applied: C-application with water; M-application with untreated C. vulgaris microalgae; M-US-application with C. vulgaris microalgae treated with ultrasound; M-USHY-application with C. vulgaris microalgae treated with ultrasound and enzymatic hydrolysis; and M-USHYAU-application with C. vulgaris microalgae treated with ultrasound, enzymatic hydrolysis, and autoclaving. All microalgae treatments increased shoot number and stem and plant diameter. The US-treated biomass increased the inflorescence of the plant significantly compared to the control. To extract bioactive compounds from eukaryoti...