A new culture medium based on genetic algorithms for Isochrysis galbana production relevant to hatcheries
Ficheros
Identificadores
URI: http://hdl.handle.net/10835/15369
ISSN: 0921-8971
DOI: https://doi.org/10.1007/s10811-021-02564-3
ISSN: 0921-8971
DOI: https://doi.org/10.1007/s10811-021-02564-3
Compartir
Metadatos
Mostrar el registro completo del ítemAutor
Camacho-Rodríguez, J.; Gallardo Rodríguez, Juan José; Cerón García, María Del Carmen; García Camacho, Francisco; Molina Grima, EmilioFecha
2021-09-04Resumen
The nutrient content of a commercial seawater culture medium for growing the microalga Isochrysis galbana was optimized using a stochastic strategy based on genetic algorithms. For this, 210 experiments spread over seven generations were carried out. This strategy reduced the number of assays by more than 90% compared to a factorial design involving the optimization of twelve nutrients simultaneously. The optimized medium outperformed the reference medium in all aspects. The genetic algorithm strategy achieved a polyunsaturated fatty acids (PUFAs) productivity of 7.8 mg L−1 day−1 in a continuous culture of I. galbana, corresponding to an increase of 15% compared to the commercial formulation. Carotenoids, on the other hand, increased by 50% d.w. In addition, PUFA yields were significantly improved, which allowed us to reduce the requirement of several nutrients, for instance, N (25%), Mo (20%), Mn (60%), Co (60%), and Cu (60%).
Palabra/s clave
Microalgae
Medium optimization
Genetic algorithm
Isochrysis galbana
Aquaculture
PUFAs
Fucoxanthin