Long-term effects of two organic amendments on bacterial communities of calcareous mediterranean soils degraded by mining
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Rodríguez-Berbel, Natalia; Ortega Pérez, Raúl; Lucas Borja, Manuel Esteban; Solé Benet, Alberto; Miralles Mellado, IsabelFecha
2020-10-01Resumen
The application of organic amendments to improve the chemical and biological properties of degraded soils from calcareous quarries is necessary to accelerate restoration processes. The aim of this study is to assess the success of different restoration treatments in the long-term using two organic amendments (sewage sludge from urban waste water (SS) and compost from domestic solid waste (CW)). The chemical properties and bacterial communities of restored soils were compared with unamended soils (NA) and surrounding natural soils (NS) from a limestone quarry in a semi-arid ecosystem. After 10 years of the addition of organic amendments, the abundance of soil bacteria, diversity, and taxonomic composition at the phylum and genus level in each soil type was analysed by rRNA 16 S amplification (PCR), sequencing using Illumina, and comparison with the SILVA database using QIIME2 software. The relationships between soil bacterial taxa and chemical soil properties (pH, electrical conductivit...
Palabra/s clave
Soil restoration
Metagenomics
Bacterial diversity
Soil degradation
Soil bacterial co-occurrence patterns
Semi-arid ecosystems