Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor
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Author/sHita Peña, Estrella; Robles Medina, Alfonso; Camacho Páez, Belén; González Moreno, Pedro Antonio; Esteban Cerdán, Luis; [et al.]
The aim of this work was to produce structured triacylglycerols (STAGs), with caprylic acid located at positions 1 and 3 of the glycerol backbone and docosohexaenoic acid (DHA) at position 2, by acidolysis of tuna oil and caprylic acid (CA) catalyzed by lipases Rd, from Rhizopus delemar, and Palatase 20000L from Mucor miehei immobilized on Accurel MP1000 in a packed bed reactor (PBR), working in continuous and recirculation modes. First, different lipase/support ratios were tested for the immobilization of lipases and the best results were obtained with ratios of 0.67 (w/w) for lipase Rd and 6.67 (w/w) for Palatase. Both lipases were stable for at least 4 days in the operational conditions. In the storage conditions (5 ºC) lipases Rd and Palatase maintained constant activity for 5 and 1 month, respectively. These catalysts have been used to obtain STAGs by acidolysis of tuna oil and CA in a PBR operating with recirculation of the reaction mixture through the lipase bed. Thus, STAGs wi...
docosohexaenoic acid (DHA)
packed bed reactor
lipase from Rhizopus delemar