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dc.contributor.authorKrock, Bernd
dc.contributor.authorBusch, Julia A.
dc.contributor.authorTillmann, Urban
dc.contributor.authorGarcía Camacho, Francisco
dc.contributor.authorSánchez Mirón, Asterio
dc.contributor.authorGallardo Rodríguez, Juan José
dc.contributor.authorLópez Rosales, Lorenzo
dc.contributor.authorAndree, Karl B.
dc.contributor.authorFernández Tejedor, Margarita
dc.contributor.authorWitt, Matthias
dc.contributor.authorCembella, Allan D.
dc.contributor.authorPlace, Allen R.
dc.date.accessioned2020-01-16T08:20:22Z
dc.date.available2020-01-16T08:20:22Z
dc.date.issued2017-12-18
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10835/7325
dc.description.abstractA liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the detection and quantitation of karlotoxins in the selected reaction monitoring (SRM) mode. This novel method was based upon the analysis of purified karlotoxins (KcTx-1, KmTx-2, 44-oxo-KmTx-2, KmTx-5), one amphidinol (AM-18), and unpurified extracts of bulk cultures of the marine dinoflagellate Karlodinium veneficum strain CCMP2936 from Delaware (Eastern USA), which produces KmTx-1 and KmTx-3. The limit of detection of the SRM method for KmTx-2 was determined as 2.5 ng on-column. Collision induced dissociation (CID) spectra of all putative karlotoxins were recorded to present fragmentation patterns of each compound for their unambiguous identification. Bulk cultures of K. veneficum strain K10 isolated from an embayment of the Ebro Delta, NW Mediterranean, yielded five previously unreported putative karlotoxins with molecular masses 1280, 1298, 1332, 1356, and 1400 Da, and similar fragments to KmTx-5. Analysis of several isolates of K. veneficum from the Ebro Delta revealed small-scale diversity in the karlotoxin spectrum in that one isolate from Fangar Bay produced KmTx-5, whereas the five putative novel karlotoxins were found among several isolates from nearby, but hydrographically distinct Alfacs Bay. Application of this LC-MS/MS method represents an incremental advance in the determination of putative karlotoxins, particularly in the absence of a complete spectrum of purified analytical standards of known specific potency.es_ES
dc.language.isoenes_ES
dc.publisherMDPIes_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectphycotoxines_ES
dc.subjectichthyotoxines_ES
dc.subjectHABes_ES
dc.subjectharmful algal bloomes_ES
dc.subjectplanktones_ES
dc.subjectAlfacs Bayes_ES
dc.subjectFangar Bayes_ES
dc.subjectMediterranean Seaes_ES
dc.titleLC-MS/MS Detection of Karlotoxins Reveals New Variants in Strains of the Marine Dinoflagellate Karlodinium veneficum from the Ebro Delta (NW Mediterranean)es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://www.mdpi.com/1660-3397/15/12/391es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional