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Optimisation of Protein Recovery from Arthrospira platensis by Ultrasound-Assisted Isoelectric Solubilisation/Precipitation
dc.contributor.author | Sánchez Zurano, Ana | |
dc.contributor.author | Morillas España, Ainoa | |
dc.contributor.author | González López, Cynthia Victoria | |
dc.contributor.author | Lafarga, Tomás | |
dc.date.accessioned | 2020-12-09T10:31:48Z | |
dc.date.available | 2020-12-09T10:31:48Z | |
dc.date.issued | 2020-12-01 | |
dc.identifier.issn | 2227-9717 | |
dc.identifier.uri | http://hdl.handle.net/10835/9029 | |
dc.description.abstract | A response surface methodology was used to optimise the solubilisation and precipitation of proteins from the cyanobacterium Arthrospira platensis. Two separate experiments were designed and conducted in a sequential manner. Protein solubilisation was affected by pH, extraction time, and biomass to solvent ratio (p < 0.001). Although spray-drying and the osmotic shock suffered when resuspending the dried biomass into distilled water led to a certain degree of cell wall disruption, the amount of protein that could be solubilised without an additional disruption step was in the range 30–60%. Sequential extractions improved protein solubilisation by less than 5%. For this reason, a pre-treatment based on sonication (400 W, 24 kHz, 2 min) had to be used, allowing the solubilisation of 96.2% of total proteins. Protein precipitation was affected by both pH and extraction time (p < 0.001). The optimised precipitation conditions, which were pH 3.89 over 45 min, led to a protein recovery of 75.2%. The protein content of the extract was close to 80%, which could be further increased by using different purification steps. The proteins extracted could be used in the food industry as technofunctional ingredients or as a source of bioactive hydrolysates and peptides for functional foods and nutraceuticals. | es_ES |
dc.language.iso | en | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | microalgae | es_ES |
dc.subject | cell wall disruption | es_ES |
dc.subject | novel proteins | es_ES |
dc.subject | Spirulina | es_ES |
dc.subject | sonication | es_ES |
dc.title | Optimisation of Protein Recovery from Arthrospira platensis by Ultrasound-Assisted Isoelectric Solubilisation/Precipitation | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2227-9717/8/12/1586 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |