Multi-analysis determination of tropane alkaloids in cereals and solanaceaes seeds by liquid chromatography coupled to single stage Exactive-Orbitrap
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2017-08Abstract
Tropane alkaloids are a wide group of substances that comprises more than 200 compounds occurring
especially in the Solanaceae family. The main aim of this study is the development of a method for the
analysis of the principal tropane alkaloids as atropine, scopolamine, anisodamine, tropane, tropine, littorine,
homatropine, apoatropine, aposcopolamine, scopoline, tropinone, physoperuvine, pseudotropine
and cuscohygrine in cereals and related matrices. For that, a simple solid-liquid extraction was optimized
and a liquid chromatographic method coupled to a single stage Exactive-Orbitrap was developed. The
method was validated obtaining recoveries in the range of 60–109% (except for some compounds in soy),
precision values (expressed as relative standard deviation) lower than 20% and detection and quantification
limits equal to or lower than 2 and 3 !g/kg respectively. Finally, the method was applied to the
analysis of different types of samples as buckwheat, linseed, soy and mi...
Palabra/s clave
Tropane alkaloids
LC-Orbitrap
Cereals
Solanaceaes seeds