Simultaneous determination of atropine and scopolamine in buckwheat and related products using modified QuEChERS and liquid chromatography tandem mass spectrometry
Identificadores
URI: http://hdl.handle.net/10835/13777
DOI: http://dx.doi.org/10.1016/j.foodchem.2016.09.075
DOI: http://dx.doi.org/10.1016/j.foodchem.2016.09.075
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2017Resumen
A method was developed for the determination of atropine and scopolamine in buckwheat and related
products. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction procedure
was evaluated. Dispersive solid phase extraction (d-SPE) was studied as clean-up step, using graphitized
black carbon (GBC) and primary secondary amine (PSA). The extract was diluted with water (50:50, v/v)
prior to chromatographic analysis. The method was validated and recoveries (except chia samples spiked
at 10 lg/kg) ranged from 75% to 92%. Intra and inter-day precision was lower than or equal to 17%. The
limit of quantification of atropine and scopolamine was 0.4 and 2 lg/kg, respectively. Eight types of
samples (buckwheat, wheat, soy, buckwheat flour, buckwheat noodle, amaranth grain, chia seeds and
peeled millet) were analyzed. Target compounds were not found above the detection limits of the
method, but three transformation products of scopolamine (norscopine, hydroscopolami...
Palabra/s clave
Atropine
Scopolamine
Buckwheat and related food
Modified QuEChERS
Liquid chromatography tandem mass spectrometry
Transformation products