Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)
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AutorSantigosa, Ester; Saénz de Rodrigáñez, Miguel Ángel; Rodiles Guerrero, Ana; García Barroso, Fernando; Alarcón López, Francisco Javier
Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g/kg of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0,15 and 30 days of dietary treatment. No signi¢cant di¡erences were found in the weight gain, speci¢c growth rate (SGR) and feed conversion rate in fish fed on inhibitor-supplemented diets when compared with those fed on an inhibitor-free diet. Only the SGR at day 15 decreased signi¢cantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in ¢sh fed on inhibitor-supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed onthe highest level of soybean inhibitor (4.0 g...