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dc.contributor.authorGil García, María Dolores
dc.contributor.authorBarranco Martínez, Dolores
dc.contributor.authorMartínez Galera, María
dc.contributor.authorParrilla Vázquez, Piedad
dc.date.accessioned2011-07-22T07:25:40Z
dc.date.available2011-07-22T07:25:40Z
dc.date.issued2004
dc.identifier.citationJournal of Separation Science 27 (2004) 1173-1180es_ES
dc.identifier.issn1615-9306
dc.identifier.urihttp://hdl.handle.net/10835/93
dc.description.abstractThe coupled-column (LC-LC) system, consisting of a first column packed with internal surface reversed phase (ISRP) (50x4.6 mm ID) and a Chrompack C18 (100x4.6 mm ID) as second column, allowed the simultaneous determination of five benzoylurea insecticides in dichloromethane (CH2Cl2) extracts of vegetable samples without any clean-up step. This system was combined with a photochemically induced fluorescence (PIF) post-column derivatization in order to provide strongly fluorescent photoproducts from the non-fluorescent benzoylureas. Limits of detection ranged from 0.21 to 0.98 ug L–1 of pesticide (equivalent to 0.14–0.65 ug kg–1 in vegetable samples) and limits of determination ranged from 4.0 to 10.0 ug L–1 (equivalent to 2.7–6.7 ug kg–1). Linearity of the method was established between 2 and 1800 ug L–1, depending upon the compound. Validation of the total method was performed by randomly analyzing recoveries of four vegetable samples (aubergine, cucumber, green bean, and tomato) spiked at two levels of concentration (10.0 and 33.3 ug kg–1). The combination of the LC-LC system with PIF detection provides a sensitive, selective, and rapid method for the determination of pesticides in vegetable samples at levels lower than the maximum residue levels (MRLs) established for these compounds by Spanish legislation.es_ES
dc.language.isoenes_ES
dc.publisherWiley-Blackwelles_ES
dc.subjectBenzoylurea insecticideses_ES
dc.subjectPhotochemically induced fluorescencees_ES
dc.subjectLC-LC separationes_ES
dc.subjectVegetableses_ES
dc.titleCoupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetableses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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