Coupling a bio-accumulator organism and MALDI-TOF MS: an early warning detection system for microcystins in water bodies
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Lauceri, Rosaria; Austoni, Martina ; Caviglia, Francesca ; Kamburska, Lyudmila ; Lami, Andrea ; [et al.]Date
2017-01-12Abstract
Microcystins produced by freshwater cyanobacteria pose serious threats to human health and are a growing problem in drinking water supplies worldwide. Toxin detection and identification in water reservoirs when cyanobacterial density is still low is a key step to predict harmful algal blooms and to allow the safe use of the resource. For this purpose, developing sensitive and rapid methods of analysis is crucial. Adopting a strategy that couples the use of a filter-feeder organism (Unio elongatulus, Pfeiffer 1825 (Bivalvia: Unionidae)) with matrix-assisted laser desorption ionization-time of flight mass spec-trometry (MALDI-TOF MS) technology, effective microcystin detection was achieved. The performances of MALDI-TOF MS, high-liquid-performance chromatography with diode array detector (HPLC-DAD) and enzyme-linked immunosor-bent assays (ELISA) test were compared. The results obtained in this study suggest that the exploitation of an efficient bio-accumulator organism, coupled with a ra...