Production of extracts with anaesthetic activity from the culture of Heterosigma akashiwo in pilot-scale photobioreactors
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URI: http://hdl.handle.net/10835/14835
DOI: https://doi.org/10.1016/j.algal.2019.101760
DOI: https://doi.org/10.1016/j.algal.2019.101760
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Astuya, Allisson; Avello, Verónica; Llanos, Alejandra; Krock, Bernd; Agurto-Muñoz, Cristian; [et al.]Date
2019-12-19Abstract
The shear-sensitive microalga Heterosigma akashiwo is known to produce brevetoxin-like compounds that are active in voltage-dependent sodium channels. In this work, we present a study on the production of anaesthetic extracts from H. akashiwo biomass obtained in low- shear bioreactors at different growth phases. The photobioreactors (PBRs) used had specific configurations and were operated in such a way as to avoid cellular damage by hydrodynamic stress. Cultures were developed in small static-control flasks and PBRs with volumes ranging from 1.5 L to 200 L. The bioactivity of the produced extracts was assessed in vitro (Neuro- 2a cell-based assay) and in vivo (Zebra fish model). Bioactivity depended slightly on the growth phase and culture system, with greater toxicity with the Neuro-2a model when stationary-phase extracts were used. Interestingly, extracts were not cytotoxic against other human cell lines. Steady production of anaesthetic bioactives was observed. In vivo anaesthetic ...