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dc.contributor.authorLópez López, María Josefa 
dc.contributor.authorJurado Rodríguez, Macarena Del Mar 
dc.contributor.authorLópez González, Juan Antonio 
dc.contributor.authorEstrella González, Maria José 
dc.contributor.authorMartínez Gallardo, María Rosa 
dc.contributor.authorToribio Gallardo, Ana Josefa 
dc.contributor.authorSuárez Estrella, Francisca 
dc.date.accessioned2024-01-10T07:49:28Z
dc.date.available2024-01-10T07:49:28Z
dc.date.issued2021-08-11
dc.identifier.urihttp://hdl.handle.net/10835/15022
dc.description.abstractComposting involves the selection of a microbiota capable of resisting the high temperatures generated during the process and degrading the lignocellulose. A deep understanding of the thermophilic microbial community involved in such biotransformation is valuable to improve composting efficiency and to provide thermostable biomass-degrading enzymes for biorefinery. This study investigated the lignocellulose-degrading thermophilic microbial culturome at all the stages of plant waste composting, focusing on the dynamics, enzymes, and thermotolerance of each member of such a community. The results revealed that 58% of holocellulose (cellulose plus hemicellulose) and 7% of lignin were degraded at the end of composting. The whole fungal thermophilic population exhibited lignocellulose-degrading activity, whereas roughly 8–10% of thermophilic bacteria had this trait, although exclusively for hemicellulose degradation (xylan-degrading). Because of the prevalence of both groups, their enzymatic activity, and the wide spectrum of thermotolerance, they play a key role in the breakdown of hemicellulose during the entire process, whereas the degradation of cellulose and lignin is restricted to the activity of a few thermophilic fungi that persists at the end of the process. The xylanolytic bacterial isolates (159 strains) included mostly members of Firmicutes (96%) as well as a few representatives of Actinobacteria (2%) and Proteobacteria (2%). The most prevalent species were Bacillus licheniformis and Aeribacillus pallidus. Thermophilic fungi (27 strains) comprised only four species, namely Thermomyces lanuginosus, Talaromyces thermophilus, Aspergillus fumigatus, and Gibellulopsis nigrescens, of whom A. fumigatus and T. lanuginosus dominated. Several strains of the same species evolved distinctly at the stages of composting showing phenotypes with different thermotolerance and new enzyme expression, even not previously described for the species, as a response to the changing composting environment. Strains of Bacillus thermoamylovorans, Geobacillus thermodenitrificans, T. lanuginosus, and A. fumigatus exhibiting considerable enzyme activities were selected as potential candidates for the production of thermozymes. This study lays a foundation to further investigate the mechanisms of adaptation and acquisition of new traits among thermophilic lignocellulolytic microorganisms during composting as well as their potential utility in biotechnological processing.es_ES
dc.language.isoenes_ES
dc.subjectculturomees_ES
dc.subjectxylanasees_ES
dc.subjectcellulasees_ES
dc.subjectligninaseses_ES
dc.subjectlaccasees_ES
dc.subjectlignin peroxidasees_ES
dc.subjectthermotolerancees_ES
dc.titleCharacterization of Thermophilic Lignocellulolytic Microorganisms in Compostinges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doihttps://doi.org/10.3389/fmicb.2021.697480


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