Analysis and evaluation of (neuro)peptides in honey bees exposed to pesticides in field conditions
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URI: http://hdl.handle.net/10835/15574
DOI: 10.1016/j.envpol.2017.12.091
DOI: 10.1016/j.envpol.2017.12.091
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Gómez Ramos, María Del Mar; Gómez Ramos, María José; Martínez Galera, María; Gil García, María Dolores; Rodríguez Fernández-Alba, AmadeoFecha
2018-04Resumen
During the last years, declines in honey bee colonies are being registered worldwide. Cholinergic pesticides and their extensive use have been correlated to the decline of pollinators and there is evidence that pesticides act as neuroendocrine disruptors affecting the metabolism of neuropeptides. However, there is a big absence of studies with quantitative results correlating the effect of pesticide exposure with changes on neuropeptides insects, and most of them are conducted under laboratory conditions, typically with individual active ingredients. In this study, we present an analytical workflow to evaluate pesticide effects on honey bees through the analysis of neuropeptides. The workflow consists of a rapid extraction method and liquid chromatography with triple quadrupole for preselected neuropeptides. For non-target analysis, high resolution mass spectrometry, multivariate analysis and automatic identification of discriminated peptides using a specific software and protein seque...
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Química ambiental