Whole genome sequencing–based analysis of tuberculosis (TB) in migrants: rapid tools for crossborder surveillance and to distinguish between recent transmission in the host country and new importations
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Abascal, Estefanía; Pérez-Lago, Laura; Martínez-Lirola, Miguel; Chiner-Oms, Álvaro; Herranz, Marta; [et al.]Fecha
2019Resumen
The analysis of transmission of tuberculosis (TB) is challenging in areas with a large migrant population. Standard genotyping may fail to differentiate transmission within the host country from new importations, which is key from an epidemiological perspective. Aim: To propose a new strategy to simplify and optimise cross-border surveillance of tuberculosis and to distinguish between recent transmission in the host country and new importations. Methods: We selected 10 clusters, defined by 24-locus mycobacterial interspersed repetitive unitvariable number tandem repeat (MIRU-VNTR), from a population in Spain rich in migrants from eastern
Europe, north Africa and west Africa and reanalysed 66 isolates by whole-genome sequencing (WGS). A multiplex-allele-specific PCR was designed to target strain-specific marker single nucleotide polymorphisms (SNPs), identified from WGS data, to optimise the surveillance of the most complex cluster. Results: In five of 10 clusters not all isolates show...
Palabra/s clave
Bacterial Typing Techniques
Minisatellite Repeats
Mycobacterium tuberculosis
Tuberculosis
Whole Genome Sequencing