Phytoprostanes and phytofurans modulate COX-2-linked inflammation markers in LPS-stimulated THP-1 monocytes by lipidomics workflow
Identificadores
URI: http://hdl.handle.net/10835/16325
DOI: 10.1016/j.freeradbiomed.2021.03.002
DOI: 10.1016/j.freeradbiomed.2021.03.002
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Campillo, María; Medina, Sonia; Fanti, Federico; Gallego Gómez, Juana Inés![Autoridad Universidad de Almería Autoridad Universidad de Almería](/themes/Mirage2/images/autoridades/autoridad.png)
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2021-05-28Resumen
Inflammation is a fundamental pathophysiological process which occurs in the course of several diseases. The
present work describes the capacity of phytoprostanes (PhytoPs) and phytofurans (PhytoFs) (plant oxylipins),
present in plant-based foods, to modulate inflammatory processes mediated by prostaglandins (PGs, human
oxylipins) in lipopolysaccharide (LPS)-stimulated THP-1 monocytic cells, through a panel of 21 PGs and PG’s
metabolites, analyzed by UHPLC-QqQ-ESI-MS/MS. Also, the assessment of the cytotoxicity of PhytoPs and
PhytoFs on THP-1 cells evidenced percentages of cell viability higher than 90% when treated with up to 100 μM.
Accordingly, 50 μM of the individual PhytoPs and PhytoFs 9-F1t-PhytoP, 9-epi-9-F1t-PhytoP, ent-16-F1t-PhytoP,
ent-16-epi-16-F1t-PhytoP, ent-9-D1t-PhytoP, 16-B1-PhytoP, 9-L1-PhytoP, ent-16(RS)-9-epi-ST-Δ14-10-PhytoF, ent-9
(RS)-12-epi-ST-Δ10-13-PhytoF, and ent-16(RS)-13-epi-ST-Δ14-9-PhytoF were evaluated on their capacity to
modulate the expressio...
Palabra/s clave
Monocytes
Phytoprostanes
Phytofurans
Inflammation
Prostaglandins
UHPLC-QqQ-ESI-MS/MS