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Structural Characterization of β-Xylosidase XynB2 from Geobacillus stearothermophilus CECT43: A Member of the Glycoside Hydrolase Family GH52
dc.contributor.author | Gavira, J.A. | |
dc.contributor.author | Contreras Moyeja, Lellys Mariela | |
dc.contributor.author | Alshamaa, Hassan Mohamad | |
dc.contributor.author | Clemente Jiménez, Josefa María | |
dc.contributor.author | Rodríguez Vico, Felipe | |
dc.contributor.author | Las Heras Vázquez, Francisco Javier | |
dc.contributor.author | Martínez Rodríguez, Sergio | |
dc.date.accessioned | 2024-05-21T07:47:18Z | |
dc.date.available | 2024-05-21T07:47:18Z | |
dc.date.issued | 2024 | |
dc.identifier.issn | 2073-4352 | |
dc.identifier.uri | http://hdl.handle.net/10835/16486 | |
dc.description.abstract | β-xylosidases (4-β-D-xylan xylohydrolase, E.C. 3.2.1.37) are glycoside hydrolases (GH) catalyzing the hydrolysis of (1→4)-β-D-xylans, allowing for the removal of β-D-xylose residues from its non-reducing termini. Together with other xylan-degrading enzymes, β-xylosidases are involved in the enzymatic hydrolysis of lignocellulosic biomass, making them highly valuable in the biotechnological field. Whereas different GH families are deeply characterized from a structural point of view, the GH52 family has been barely described. In this work, we report the 2.25 Å resolution structure of Geobacillus stearothermophilus CECT43 XynB2, providing the second structural characterization for this GH family. A plausible dynamic loop closing the entrance of the catalytic cleft is proposed based on the comparison of the available GH52 structures, suggesting the relevance of a dimeric structure for members of this family. The glycone specificity at the −1 site for GH52 and GH116 members is also explained by our structural studies. | es_ES |
dc.language.iso | en | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | crystallization of β-xylosidase | es_ES |
dc.subject | XynB2 | es_ES |
dc.subject | Glycoside Hydrolase Family 52 | es_ES |
dc.subject | Glycoside Hydrolase Family 116 | es_ES |
dc.title | Structural Characterization of β-Xylosidase XynB2 from Geobacillus stearothermophilus CECT43: A Member of the Glycoside Hydrolase Family GH52 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |