Carbamoylases: characteristics and applications in biotechnological processes
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Martínez Rodríguez, Sergio; Martínez Gómez, Ana Isabel; Rodríguez Vico, Felipe; Clemente Jiménez, Josefa María; Las Heras Vázquez, Francisco JavierFecha
2010Resumen
Abstract Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-D and L-amino acid amidohydrolases (D- and L-carbamoylases) are model enzymes for this procedure due to their strict enantiospecificity. Carbamoylase-based kinetic resolution of amino acids has been applied for the last three decades, allowing the production of optically pure D- or L-amino acids. Furthermore, this enzyme has become crucial in the industrially used multienzymatic system known as “Hydantoinase Process,” where the kinetic resolution produced by coupling an enantioselective hydantoinase and the enantiospecific carbamoylase is enhanced by the enzymatic/chemical dynamic kinetic resolution of the low-rate hydrolyzed substrate. This review outlines the prop...
Palabra/s clave
L-Carbamoylase
N-Carbamoyl-L-amino acid amidohydrolase
D-Carbamoylase
N-Carbamoyl-D-amino acid amidohydrolase
Hydantoinase Process
Ureidopropionase
Peptidase family M20/M25/M40
Nitrilase family
Kinetic resolution