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Optically pure a-amino acids production by the Hydantoinase Process
dc.contributor.author | Clemente Jiménez, Josefa María | |
dc.contributor.author | Martínez Rodríguez, Sergio | |
dc.contributor.author | Rodríguez Vico, Felipe | |
dc.contributor.author | Las Heras Vázquez, Francisco Javier | |
dc.date.accessioned | 2024-05-23T09:56:06Z | |
dc.date.available | 2024-05-23T09:56:06Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 2212-4012 | |
dc.identifier.uri | http://hdl.handle.net/10835/16552 | |
dc.description.abstract | Optically pure D- or L-amino acids are used as intermediates in several industries. D- aminio acids are involved in the synthesis of antibiotics, pesticides, sweeteners and other biologically active peptides. L-amino acids are used as feed and food additives, as intermediates for pharmaceuticals, cosmetics, pesticides and as chiral synthons in organic synthesis. The specific activity of these optically pure amino acids depends on their structure, chirality and purity. There are two main approaches to obtain optically pure amino acids, namely chemical and enzymatic synthesis. Chemical synthesis gives racemicc mixtures of amino acis of low yield and is not environment friendly. One of the most widely-used enzymatic method is the "Hydanotinase Process". In this cascade of reactions, the chemically synthesized D,L-5-monosubstituted hydantoin ring is first hydrolyzed by a stereoselective hydantoinase enzyme to give the corresponding N-carbamoyl a-amino acid that is hydrolyzed by hinghly enantiospecific N-carbamoyl a-amino acid amidohydrolase (N-carbamoylase) to yield the free amino acid. At the same time, the remaining nong-hydrolyzed 5-monosubstituted hydantoin is racemized by the hydantoin racemase enzyme. This process has evolved over the years from the isolation of micoorganisms with one or several of these enzymes to the construction of recombinant systems for industrial application. | es_ES |
dc.language.iso | en | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Hydantoinase process | es_ES |
dc.title | Optically pure a-amino acids production by the Hydantoinase Process | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |