TY - GEN AU - Andújar Sánchez, Montserrat AU - Las Heras Vázquez, Francisco Javier AU - Clemente Jiménez, Josefa María AU - Martínez Rodríguez, Sergio AU - Cámara Artigas, Ana María AU - Rodríguez Vico, Felipe AU - Jara Pérez, Vicente PY - 2006 SN - 0165-022X UR - http://hdl.handle.net/10835/16574 AB - Isothermal titration calorimetry (ITC) has been applied to the determination of the activity of dhydantoinase (EC 3.5.2.2) with several substrates by monitoring the heat released during the reaction. The method is based on the proportionality between... LA - en KW - d-Hydantoinase KW - Isothermal titration calorimetry KW - Activity assay KW - Activation parameters TI - Enzymatic activity assay of d-hydantoinase by isothermal titration calorimetry. Determination of the thermodynamic activation parameters for the hydrolysis of several substrates ER -