A step forward in sustainable pesticide production from Amphidinium carterae biomass via photobioreactor cultivation with urea as a nitrogen source
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URI: http://hdl.handle.net/10835/15231
ISSN: 0960-8524
DOI: https://doi.org/10.1016/j.biortech.2023.129643
ISSN: 0960-8524
DOI: https://doi.org/10.1016/j.biortech.2023.129643
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Molina Miras, Alejandro; Ralha Abreu, Ana Cristina; López Rosales, Lorenzo; Cerón García, María Del Carmen; Sánchez Mirón, Asterio; [et al.]Fecha
2023-08-05Resumen
This study addresses the problem of replacing nitrate and ammonium with urea as a greener nitrogen source in the mass cultivation of the microalga Amphidinium carterae for the development of amphidinol-based phytosanitary products. To solve this problem, a nuclear magnetic resonance assisted investigation evaluated the effect of nitrogen sources on growth and metabolic profiles in photobioreactors. Urea-fed cultures exhibited growth kinetics comparable to nitrate-fed cultures (µmax = 0.30 day−1, Pbmax = 43 mgL-1day−1). Urea-fed cultures had protein, lipid, and carbohydrate contents of 39.5%, 14.5%, and 42.4%, respectively, while nitrate-fed cultures had 27.9 %, 17.5% and 48.1%, respectively. Metabolomics revealed nitrogen source-dependent metabotypes and a correlation between amphidinols and polyunsaturated fatty acids. The amphidinol-to-nitrogen yield coefficient in urea-fed cultures (135 mg/g) was approximately 2.5 times higher than in nitrate-fed cultures. The potent antiphytopathog...
Palabra/s clave
microalgae
dinoflagellate
metabolomics
amphidinol
NMR