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Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia
dc.contributor.author | Merchán Carrillo, Ana María | |
dc.contributor.author | Pérez Fernández, Cristian Antonio | |
dc.contributor.author | López López, María José | |
dc.contributor.author | Moreno Casco, José Joaquín | |
dc.contributor.author | Moreno Montoya, Margarita Begoña | |
dc.contributor.author | Sánchez Santed, Luis Fernando | |
dc.contributor.author | Flores Cubos, María Del Pilar | |
dc.date.accessioned | 2024-01-19T13:21:20Z | |
dc.date.available | 2024-01-19T13:21:20Z | |
dc.date.issued | 2021-02-10 | |
dc.identifier.citation | Merchán, A., Pérez-Fernández, C., López, M., Moreno, J., Moreno, M., Sánchez-Santed, F., & Flores, P. (2021). Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia. Physiology & Behavior, 233, 113356. https://doi.org/10.1016/j.physbeh.2021.113356 | es_ES |
dc.identifier.issn | 00319384 | |
dc.identifier.uri | http://hdl.handle.net/10835/15264 | |
dc.description.abstract | Rationale: Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global 5-H synthesis in the enteric and central nervous systems, suggesting a possible involvement of gut microbiota in compulsive spectrum disorders. Objectives: The present study investigated whether chronic TRP depletion by diet alters the faecal bacterial community profiles of compulsive versus non-compulsive rats in schedule-induced polydipsia (SIP). Peripheral plasma 5-HT and brain-derived neurotrophic factor (BDNF) levels were evaluated. Methods: Wistar rats were selected as High Drinkers (HD) or Low Drinkers (LD) according to their SIP behaviour and were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+). The faecal bacterial community structure was investigated with 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis. Results: Compulsive HD rats showed a lower bacterial diversity than LD rats, irrespectively of the diet. The TRP-depleted HD rats, the only group increasing compulsive licking in SIP, showed a reduction of bacterial evenness and a highly functionally organized community compared with the other groups, indicating that this bacterial community is more fragile to external changes due to the dominance of a low number of species. The chronic TRP depletion by diet effectively reduced peripheral plasma 5-HT levels in both HD and LD rats, while plasma BDNF levels were not altered. Conclusions: These results highlight the possible implication of reduced microbial diversity in compulsive behaviour and the involvement of the serotonergic system in modulating the gut brain-axis in compulsive spectrum disorders. | es_ES |
dc.language.iso | en | es_ES |
dc.publisher | Thomas Lutz | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.source | Volume 233, 1 May 2021, 113356 | es_ES |
dc.subject | Compulsivity | es_ES |
dc.subject | Schedule-induced polydipsia | es_ES |
dc.subject | Gut microbiota | es_ES |
dc.subject | Chronic tryptophan depletion | es_ES |
dc.subject | PCR-DGGE | es_ES |
dc.subject | Brain-derived neurotrophic factor | es_ES |
dc.title | Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | https://doi.org/10.1016/j.physbeh.2021.113356 | |
dc.relation.projectID | PGC2018–099,117-B-C21 | es_ES |